Phospho irf3 antibody

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August undefined, 2024

WebThis Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-IRF3 (S386) and total IRF3. An anti-pan IRF3 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and IRF3 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. WebThe phospho-IRF3 (Ser386) assay measures IRF3 when phosphorylated at Ser386. Contrary to Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis or transfer. The phospho-IRF3 (Ser386) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor.

Phospho-IRF-3 (Ser396) Antibody Cell Signaling …

WebInvitrogen Anti-Phospho-IRF3 (Ser385) Polyclonal, Catalog # PA5-36775. Tested in Western Blot (WB) and Immunohistochemistry (Paraffin) (IHC … WebThe wells are washed and rabbit anti-phospho-IRF3 (S386) antibody is used to detect phosphorylated IRF3. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IRF3 (S386) bound. greedy pig game online

Phospho-IRF3 (Ser396) Monoclonal Antibody (E.875.8)

WebPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Preparation. The antibody was purified by affinity chromatography. Concentration. 0.5 mg/ml. Storage & Handling. The antibody solution should be stored undiluted between 2°C and 8°C. Application. WB - Quality tested. WebIRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. WebAnti-IRF3 (phospho S386) antibody [EPR2346] (ab76493) Research with confidence – consistent and reproducible results with every batch. Long-term and scalable supply – powered by recombinant technology for fast … greedy pig farnborough

Cells Free Full-Text cGAS Regulates the Radioresistance of …

WebMouse liver lysates probed with Anti-IRF3 (Ser396) Polyclonal Antibody, Unconjugated (bs-3195R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C. ... Rat Heart tissue lysates; Lane 4: Rat Testis tissue lysates probed with Phospho-IRF3 (Ser396) Polyclonal Antibody ... WebJun 1, 2024 · IRF-3 Antibody (SL-12) is an IgG 1 κ mouse monoclonal IRF-3 antibody (also designated IRF3 antibody) that detects the IRF-3 protein of mouse, rat and human origin … greedy pig chance gameWebIRF3 Antibody detects endogenous levels of total IRF3 protein. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthtic peptide corresponding to a residues surrounding proline 307 of human IRF-3. Antibodies are purified by protein A and peptide affinity chromatography. flour bakery boston washington st

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Phospho irf3 antibody

Phospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb (Alexa Fluor

WebPhospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb (PE Conjugate) recognizes endogenous levels of IRF-3 protein only when phosphorylated at Ser396. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser396 of human IRF-3 protein. WebPhospho-IRF-3 (Ser396) Antibody detects endogenous levels of IRF-3 only when phosphorylated at Ser396. This antibody does not cross-react with other family members at physiological conditions. Species Reactivity: Human. Species predicted to react based on …

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WebThis Dot Blot data was generated using the same anti-phospho IRF3 S386 antibody clone, EPR2346, in a different buffer formulation (cat# ab76493). Dot blot analysis of IRF3 single phospho peptide pS386 (lane 1) and IRF3 non-phospho peptide (lane 2) with ab76493 at 1/1000. Blocking and diluting buffer was 5% NFDM/TBST. The secondary antibody used ... WebPhospho-IRF3 (Ser385) Antibody detects endogenous levels of IRF3 only when phosphorylated at Serine 385. RRID: AB_2846808 Cite Format: Affinity Biosciences Cat# AF3910, RRID:AB_2846808. Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho …

WebAnti-IRF3 (phospho S386) antibody [EPR2346] from Abcam Supplier Page WebIntracellular Delivery of Lipopolysaccharide Induces Effective Th1-Immune Responses Independent of IL-12 Sachiko Watanabe1., Joe Inoue1,2*. 1Department of Biosciences, School of Science and Graduate School of Science, Kitasato University, Japan, 2Department of Immunology and Cell Biology, Graduate

WebKey features and details Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply Rabbit monoclonal [EPR2346] to IRF3 (phospho S386) - BSA and Azide free … WebA rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. It is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome.

WebICC/IF analysis of PFA-fixed HepG2 cells (30min of 4μM Forskolin treatment) using GTX02883 IRF3 (phospho Ser396) antibody. Red : Primary antibody Green : Beta tubulin Blue : DAPI Permeabilization : 0.1% Triton X-100 GTX02883 IHC-P Image IHC-P analysis of mouse heart tissue using GTX02883 IRF3 (phospho Ser396) antibody. ...

WebThis Cell Signaling Technology antibody is conjugated to Alexa Fluor ® 647 fluorescent dye and tested in-house for direct flow cytometric analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb #29047. Product Usage Information Storage greedy piggish dan wordWebI haven't been able to detect human phospho-IRF3 (p-IRF3) on Wester Blot yet. The protocol I used: Block with blocking buffer for 1 hour. I tried blocking in 5% milk in TBST and in 0.5% BSA... greedy pig game board greedy pig eccleshillWebThis antibody is predicted to react with Monkey. Target Information IFR3 (interferon regulatory factor 3) is a key transcriptional regulator of type I interferon (IFN)-dependent … greedy pig imageWebICC/IF analysis of PFA-fixed HepG2 cells (30min of 4μM Forskolin treatment) using GTX02883 IRF3 (phospho Ser396) antibody. Red : Primary antibody Green : Beta tubulin … greedy piggish crossword clue dan wordWebPhospho-IKK gamma (Ser85) Antibody detects endogenous levels of IKK gamma only when phosphorylated at Serine 85. RRID: AB_2834791 引用格式: Affinity Biosciences Cat# AF3496, RRID:AB_2834791. ... Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked ... flour bakery gift card balanceWebMar 16, 2024 · Anti-Phospho-IRF3 (Ser386) Antibody (PACO24125) from Assay Genie is a high quality polyclonal antibody developed for research and validated in WB applications Toggle menu Select Currency: EUR greedy piggish