Smad3 western blot
WebbIt was further verified in relative cell lines by real-time quantitative polymerase chain reaction and Western blotting (WB). Adenocarcinoma cell lines were transfected with si-NC and si- AHNAK2 by lipofectamine 3000 and treated with or without TGF-β 1, and cell migration and invasion were detected by wound-healing and transwell assays. WebbThis study was to observe effects of puerarin on expression of cardiac transforming growth factor β1 (TGF-β1), Smad3 and Smad7 mRNA in the spontaneously hypertensive rat (SHR), and to explore its possible mechanism of myocardial protection.Materials and methodsThirty-five 12-week-old SHRs were randomly allocated into 5 groups of 7 rats as …
Smad3 western blot
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WebbUpon TGF-β1 stimulation, knockdown of SMAD3 or/and SMAD4 led to a significant reduction in CDH2 promoter activity, and silencing of SMAD3 or SMAD4 significantly … WebbProtein levels of endogenous Smad3 were analyzed by Western blot and quantified on the right. Smad3 level in the KO cells at time “0” was defined as 1.0. (B) Specific knockdown …
Webb10 feb. 2024 · Western blot validates the inflammatory signaling pathway action of inflammatory response and biomarkers. EGCG was shown to decrease the protein expression of NF-κB2 ( Figure 15 A,B and Supplementary Figure S1 ), Akt3 ( Figure 16 A,B and Supplementary Figure S2 ), and mTOR ( Figure 17 A,B and Supplementary Figure S3 ). WebbWestern blot analysis of SMAD3 (Fig. a) was performed by loading 20 µg of HeLa Control (lane 1), HeLa SMAD3 knockout (lane 2) whole cell extracts. SMAD3 was detected at 53 …
WebbSMAD3 Antibodies. Antibodies that detect SMAD3 can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, … Webb13 apr. 2024 · SM reduced relative protein and gene expression in mice peritoneal fibrosis (PF) by RT-qPCR and Western blotting respectively ( n = 6/group). a – d the mRNA expression of TGF-β1, Smad2, Smad3, Smad4, and smad7 in the peritoneal tissue. e Protein bands of TGF-β1, p-Smad2, Smad2, p-Smad3, Smad3, Smad4, Smad7, and GAPDH. f – l …
WebbWestern blot analysis also confirmed that Smad3 / mice failed to express Smad3 in the kidney after either sham ischemia or renal ischemia, thereby confirm- ing the deficiency …
WebbRT-PCR and western blot shows the same trend, refer to Figures 4 and 5 for details. Figure 3. ... CRP promotes renal fibrosis and inflammation through a TGF-β/SMad3-dependent mechanism. 31 In our study, the serum hs-CRP level was significantly increased in CKD patients with SHPT. chitty chitty bang bang lengthWebbWestern Blot (WB) 1:500-1:2000 - Visit thermofisher.com to view references and figures. Products are warranted to operate or perform substantially in conformance with published Product specifications in effect at the time of sale, as set forth in the Production documentation, specifications and/or accompanying package inserts ("Documentation"). chitty chitty bang bang kids namesWebbCompare SMAD3 antibody orb535103 from Biorbyt on Biocompare.com. Welcome Guest. Sign In Register. Products. Popular Categories; ... This antibody has been shown to work in applications such as: Western Blot. The SMAD3 antibody was generated using SMAD3 as the antigen and it reacts with Human. Description Rabbit polyclonal antibody to SMAD3 ... grasshopper 329 parts manualWebbAnti-phospho-Smad3 (pSer204) antibody produced in rabbit has been used in western blot analysis.(7) Biochem/physiol Actions Smad3 (mothers against decapentaplegic … grasshopper 335 mower partsWebb5 feb. 2024 · Total protein samples were extracted from the cells and equal amounts of total protein (20 μg) were subjected to Western blotting analysis. The levels of indicated proteins were assessed by a standard immuno-blotting technique with a specific primary antibody. Actin indicates equal amounts of protein loading into each lane (left panel). grasshopper 329b manualWebbHuman Phospho-Smad2/3 (S465/S467) Antibody Summary Species Reactivity Human Specificity Detects human Smad2 and Smad3 when dually phosphorylated at S465 and … grasshopper 325d parts manualWebbThe Total-SMAD3 assay quantifies the expression level of SMAD3 in a cell lysate. Contrary to Western Blot, the assay is entirely plate-based and does not require gels, … grasshopper 329b price