Spi1 fission yeast pombe gfp localization

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August undefined, 2024

WebResults Dephosphorylation of Hog1 is not required for nuclear export during adaptation. To examine the effect of Hog1 dephosphorylation on its localization, we produced a strain expressing Hog1–GFP from a low-copy plasmid in ahog1Δ background. To quantify the features of this process, we determined the percentage of cells having greater nuclear … WebSep 7, 2010 · To address these questions we performed a systematic analysis of the 20 DUBs in the fission yeast Schizosaccharomyces pombe using confocal microscopy, ... GFP localization was most severely altered in the case of DUBs that we and others have shown to be part of protein complexes, such as Ubp5, Rpn11, Uch2, and Ubp9. ...

Nup124p Is a Nuclear Pore Factor of Schizosaccharomyces pombe …

WebFeb 18, 2015 · All aneuploid yeast strains analyzed to date harbor elevated levels of Rad52-GFP foci, a sign of DNA damage. Here we in... Mitotic entry in the presence of DNA … Webon their localization patterns (Burns et al. 1994); in the ﬁssion yeast Schizosaccharomyces pombe a GFP-fusion genomic library that had been constructed utilizing the inducible nmt1 promoter, was ﬁrst screened for DNA fragments which affected mitotic cell growth when expressed from the nmt1 promoter. These selected barbara ewing obituary

IJMS Free Full-Text N-Terminus Does Not Govern Protein …

WebEnter the email address you signed up with and we'll email you a reset link. WebIn both untreated spi1 + or spi1-25 strains, the GFP-Pap1p fusion protein is actively exported to the cytoplasm and relocalizes to the nucleus upon oxidative stress ( Fig. 4E and Fig. F ), … WebNov 16, 1998 · We have investigated the localization of this complex in the fission yeast, Schizosaccharomyces pombe. Immunofluorescence microscopy shows a striking localization pattern whereby the proteasome is found predominantly at the nuclear periphery, both in interphase and throughout mitosis. barbara ewing husband

Localization of gene products using a chromosomally tagged GFP-fusion ...

Identification and characterization of srp1, a gene of fission yeast ...

WebDownload scientific diagram Localization of endogenous Spi1 and toxicity of Spi1-GFP in Nup107-120 complex nucleoporin mutants. (A) ⌬ nup120 cells grown at 25 ° C or shifted … Webcontributes to its localization. In this study, we investigated whether a similar mechanism functions in the fission yeast Schizosaccharomycespombe.First,weidentifiedgpp74+ (GPP34domain-containingVps74homologprotein),ageneencoding the S. pombe homolog of S. cerevisiae Vps74p. Deletion of the gpp74+ gene resulted in the missorting of three … barbara ewingWebfurther investigated, and we ﬁnd cytoplasmic Sum1 localization to be dependent on the 26S proteasome. Furthermore, Sum1 interacts with the Mts2 and Mts4 components of the 26S protea-some. These data indicate a functional link between components of the structurally related eIF3 translation initiation and 26S proteasome complexes. INTRODUCTION barbara exner uni stuttgart

"WebUnexpectedly, microscopic analyses of some of the S. pombe mutants revealed cell division defects at the restrictive temperature (abnormal septa and mitotic spindles and … " - Spi1 fission yeast pombe gfp localization

Spi1 fission yeast pombe gfp localization

Switching genes in Schizosaccharomyces pombe SpringerLink

WebMar 7, 2024 · The fission yeast Schizosaccharomyces pombe is also thought to undergo isogamous mating, but recent work revealed unexpected differences between the mating types at late stages of the mating process [19,49,50]. Thus, differences between the pheromones may be important for such late events, rather than for conveying spatial … WebNov 15, 2002 · The localization of GFP signal was observed at 0, 0.5, and 1 hour after the addition of the drug (Kudo et al., 1999). Cytologic observations Procedures for …

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WebJan 1, 1998 · We have now cloned from the fission yeast Schizosaccharomyces pombe the gene srp1. This gene is the first yeast gene encoding a protein with typical features of … WebSep 11, 2024 · There are >40 known yeast endocytic proteins with distinct spatial and temporal localizations and functions; however, it is still unclear how these proteins work together to drive endocytosis. Here, we used quantitative live-cell imaging to determine the function of the uncharacterized S. pombe protein Bbc1.

WebDownload scientific diagram Localization of endogenous Spi1 and toxicity of Spi1-GFP in Nup107-120 complex nucleoporin mutants. (A) ⌬ nup120 cells grown at 25 ° C or shifted for 4 h to 36 ... WebSep 12, 2024 · Identification of the fission yeast VPS74 homolog. Examination of the S. pombe gene database revealed that a single gene (SPAC5D6.13/vps74 +) on chromosome I predicted to encode a homolog of S. cerevisiae VPS74 (Fig. 1a).The SPAC5D6.13/vps74 + gene encodes a protein that is evolutionarily conserved from yeasts to humans belonging …

WebMar 1, 2024 · GFP-binding protein (or GBP) has been recently developed in various systems and organisms as an efficient tool to purify GFP-fusion … WebOct 1, 1998 · We expected that Rop1At would be localized to polar sites if its function is to regulate polarized growth in fission yeast. We investigated the subcellular localization of Rop1At in living yeast cells using jellyfish GFP. The GFP-Rop1At fusion gene is expressed in fission yeast under the control of the thiamine-repressiblenmt1 promoter.

WebApr 13, 2024 · We generated a tfs1-GFP strain by tagging GFP at the C-terminus of Tfs1 to visualize its localization. Tfs1-GFP localized to the nucleus and translocated into the …

WebNational Center for Biotechnology Information barbara ewing brassWebFeb 25, 2005 · In fission yeast, the Ran GTPase (Spi1p) and its regulators are essential for cell cycle progression. When the Ran GTPase is misregulated, S. pombe cells arrest after … barbara ewing draculaWebMar 1, 2024 · This series of vectors should help to facilitate the application of the GBP-targeting approach in manipulating protein localization and the analysis of gene function … barbara exnerWebMar 11, 2008 · In the fission yeast Schizosaccharomyces pombe, cell growth takes place exclusively at both ends of the cylindrical cell. During this highly polarized growth, microtubules are responsible for the placement of the cell-end marker proteins, the Tea1-Tea4/Wsh3 complex, which recruits the Pom1 DYRK-family protein kinase. barbara ewing iota louisianaWebAug 26, 2024 · It was reported previously that heterochromatin is required to establish SpCENP-A chromatin at the fission yeast centromere [30,31], and that localization of SpCENP-A to centromeres is disrupted in cells expressing the null mutant of the histone H3 lysine 9 methyltransferase Clr4 (Δclr4) , which have compromised constitutive … barbara ewing photosWebThe underlying mutations map either in a switching signal at matl or in switching ( swi) genes which are not linked to the mating-type region. Forty-nine swi mutants were … barbara f grimesWebJan 19, 2009 · We constructed a library of chromosomally‐tagged green fluorescent protein (GFP) fusions in the fission yeast Schizosaccharomyces pombe. This library contains … barbara exner hamburg