WebbThe aim of this study is to evaluate the prognostic role of thymidine labeling index in patients with breast cancer. Cellular proliferation rates in 155 breast cancer specimens … WebbSummary The 3H-thymidine labelling index (3H-dT LI) ofcell suspensions from fresh material and the flow cytometric S-phase (FCM-S)ofnuclei recovered from paraffin …
Thymidine labeling index, flow cytometric S-phase measurement, …
Thymidine-labeling indexing is determined by counting the number of tumor nuclei labeled on autoradiographed sections after incubating the tumor cells with thymidine. Rapidly proliferating cells readily update more radiolabeled Thymidine, which produces darker spots on the autoradiograph film. Visa mer Proliferation, as one of the hallmarks and most fundamental biological processes in tumors, is associated with tumor progression, response to therapy, and cancer patient survival. Consequently, the evaluation of a tumor … Visa mer Mitotic indexing is the oldest method of assessing proliferation and is determined by counting the number of mitotic figures (cells undergoing … Visa mer Evaluating DNA histograms through flow cytometry provides an estimate of the fractions of cells within each of the phases in the cell cycle. Cell nuclei are stained with a DNA … Visa mer The various methods of characterizing the proliferation index have found roles in both the diagnostic and prognostic evaluation of tumors. For instances, the number of mitotic cells is used … Visa mer A bromodeoxyuridine assay, similar to thymidine-labeling indexing, incubates cells with radiolabeled bromodeoxyuridine, which is taken up at a greater rate by proliferating cells, and then uses a film to image the distribution of radiolabeled bromodeoxyuridine … Visa mer The immunohistochemical detection of proliferation related proteins such as Ki-67 and proliferating cell nuclear antigen is a commonly used method to determine the proliferation index. Ki-67 is a nuclear antigen expressed in proliferating cells that is coded by the … Visa mer • Proliferative index entry in the public domain NCI Dictionary of Cancer Terms This article incorporates public domain material from Dictionary of Cancer Terms. U.S. National Cancer Institute Visa mer Webb14 jan. 2024 · Scientific Reports - In vivo tracking of 14C thymidine labeled mesenchymal stem cells using ultra-sensitive accelerator mass spectrometry Skip to main content … cistern\u0027s 26
Thymidine labeling index and Ki-67 growth fraction in lesions of …
WebbLabeling index measurements. The labeling index of cells in Matrigel threads after 6 d growth and the grain density of labeled cells were measured using a 3H-thymidine pulse labeling and autoradiography (Freyer and Suth-erland, 1980). Threads were labeled with 20 uCi/ml [H-3] thymidine (specific 1000 (A) Thread Diameter Thread Diameter 9L (B ... WebbThe thymidine labeling index (TLI) showed significantly higher median values in the cancer patients (3.48 per cent) than in the patients with benign diseases (1.02 per cent). TLI … WebbThe percentages of cells labelled with [^Hjthymidine (labelling index) and in mitosis (mitotic index) after each of the three pulses and the percentages of cells in metaphase after i and 3 h in colchicine after one and two pulses of non-radioactive thymidine at the labelling concentration are given in Table i. Table 2 sets out the durations of the diamond wand sky factory 3